Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ABCB11 in samples. An antibody specific for ABCB11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ABCB11 is an ABC transporter named BSEP (Bile Salt Export Pump), or sPgp (sister of P-glycoprotein). This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).This protein is a member of the MDR/TAP subfamily. Some members of the MDR/TAP subfamily are involved in multidrug resistance. This particular protein is responsible for the transport of taurocholate and other cholate conjugates from hepatocytes (liver cells) to the bile. In humans, the activity of this transporter is the major determinant of bile formation and bile flow.
