Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ABCC2 in samples. An antibody specific for ABCC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ABCC2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).his protein is a member of the MRP subfamily, which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells.Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
