Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ACAP1 in samples. An antibody specific for ACAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ACAP1 and ACAP2 were recruited to platelet-derived growth factor (PDGF)-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. The deduced protein contains 740 amino acids and shares 52% sequence identity with CENTB2. highest expression in spleen, thymus, and peripheral blood leukocytes, intermediate expression in lung, testis, and small intestine, and weak expression in prostate, ovary, and colon. No expression was detected in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, and no expression was detected in HeLa cells.ACAP1 shares significant similarity with CENTB2, which the authors called ACAP2, and 95% identity with mouse Acap1.
