Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ACPL2 in samples. An antibody specific for ACPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ACPL2 belongs to the histidine acid phosphatase family. Catalyses the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum.
Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function; therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer.
