Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ACSM3 in samples. An antibody specific for ACSM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By Southern analysis of somatic cell hybrids, Szpirer et al. (1993) independently assigned the Sa gene to rat chromosome 1 and showed that the human homolog, SAH, resides on chromosome 16. the deduced amino acid sequence from the isolated human SAH cDNA consisted of 578 amino acid residues and had slight homology to a bacterial enzyme, acetyl-CoA synthase. With the restriction enzyme PstI, they found a RFLP in the SAH gene and compared allele frequencies between hypertensive and control groups. The hypertensive group consisted of 89 persons, and the PstI rare allele (allele A2) frequency in this group was 0.270. The control group consisted of 81 healthy normotensive persons, among whom the A2 allele frequency was 0.09. The differences were significant at the P = 0.0001 level.
