Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ACTG2 in samples. An antibody specific for ACTG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Actin, gamma-enteric smooth muscle is a protein encoded by the ACTG2 gene.Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified.
The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues.
