Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ACTL6A in samples. An antibody specific for ACTL6A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTL6A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTL6A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTL6A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ACTL6A encodes a family member of actin-related proteins (ARPs), The 429-amino acid BAF53 protein shares extensive amino acid homology with actin and the yeast actin-related proteins Act2 and Act3. In addition, sequences encoding BAF53 homologs were identified in yeast and C. elegans. Two of 3 amino acids (asp11, gln137, and asp154 in actin) involved in tight calcium binding are conserved (asp17 and asp171 in BAF53).
In contrast with the overall homology to actin, the ATP-binding pocket in actin is poorly conserved in BAF53, suggesting that BAF53 may not have ATP-binding activity. A comparison of the sequences of actin family members revealed that BAF53 is most related to Arp3, which is involved in Listeria motility.
