Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADAMTS7 in samples. An antibody specific for ADAMTS7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ADAMTS7 is a member of the ADAM protein family . The gene is expressed in human brain, heart, lung, liver, pancreas, kidney, skeletal muscle, and placenta. The murine protein is expressed at low levels throughout mouse development.
The predicted 997-amino acid ADAMTS7 protein has a domain structure characteristic of ADAMTSs.
The mature ADAMTS7 protein has 2 potential N-linked glycosylation sites. Northern blot analysis detected a 5-kb ADAMTS7 transcript in all human tissues examined, namely brain, heart, lung, liver, pancreas, kidney, skeletal muscle, and placenta; skeletal muscle expressed an additional 7-kb transcript. Mouse Adamts7 was expressed at low levels throughout mouse development.
