Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADCY9 in samples. An antibody specific for ADCY9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase, and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin, calcium, and somatostatin.Like mouse Adcy9, the predicted human ADCY9 protein contains 12 transmembrane domains, Asn-linked glycosylation sites, and cAMP-dependent protein kinase phosphorylation sites; however, these proteins differ in the C2b domain due to a frameshift in the human ADCY9 coding sequence relative to the coding sequence of mouse Adcy9. Northern blot analysis detected 8.5- and 6.3-kb ADCY9 transcripts in all human tissues examined.
