Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADFP in samples. An antibody specific for ADFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Adipose differentiation-related protein, also known as ADFP or adipophilin, is a protein encoded by the ADFP gene.Adipocyte differentiation-related protein is associated with the globule surface membrane material.
This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation
Perilipin is part of a gene family with five currently-known members. In vertebrates, closely related genes include adipophilin (also known as adipose differentiation-related protein), TIP47, and LSDP5 (also called MLDP and OXPAT). Insects express related proteins, LSD1 and LSD2, in fat bodies.
