Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADH in samples. An antibody specific for ADH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Arginine vasopressin is a hormone found in most mammals, including humans. Vasopressin is a peptide hormone. It is derived from a preprohormone precursor that is synthesized in the hypothalamus and stored in vesicles at the posterior pituitary. Most of it is stored in the posterior pituitary to be released into the blood stream; however, some of it is also released directly into the brain.One of the most important roles of AVP is to regulate the body's retention of water; it is released when the body is dehydrated and causes the kidneys to conserve water, thus concentrating the urine, and reducing urine volume. In high concentrations, it also raises blood pressure by inducing moderate vasoconstriction. In addition, it has a variety of neurological effects on the brain, having been found, for example, to influence pair-bonding in voles.
