Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADH1B in samples. An antibody specific for ADH1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ADH2 encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Previously ADH1B was called ADH2. There are more genes in the family of alcohol and aldehyde dehydrogenase genes. These genes are now referred to as ADH1A, ADH1C, and ADH4, ADH5, ADH6 and ADH7.
