Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADH6 in samples. An antibody specific for ADH6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alcohol dehydrogenase 6 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products.
This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. The deduced amino acid sequence of the open reading frame of this gene shows about 60% positional identity with other known alcohol dehydrogenases. This gene may have a distinct physiologic function.The ADH6 sequence shows approximatley 60% positional identity with known human ADHs, suggesting interclass similarity within the human ADH family.
