Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADORA3 in samples. An antibody specific for ADORA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADORA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The receptor is an adenosine receptor, but also denotes the human gene encoding it.Adenosine A3 receptors are G protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. It mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death.An adenosine A3 receptor agonist (CF-101) is in clinical trials for the treatment of rheumatoid arthritis. In a mouse model of infarction the A3 selective agonist CP-532,903 protected against myocardial ischemia and reperfusion injury.
