Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate AEBP1 in samples. An antibody specific for AEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AEBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:AEBP1 binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells. The deduced 1,158-amino acid ACLP protein contains a putative signal peptide; an 11-residue lys/pro-rich motif repeated 4 times in the N terminus; a domain with 30% identity to the slime mold adhesion protein discoidin I; and a C-terminal 500-amino acid domain with 39% identity to carboxypeptidase E (CPE).
