Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate QRFP in samples. An antibody specific for QRFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:P518 functions as a high-affinity ligand of GPR103. Both GPR103 and P518 precursor mRNA exhibited highest expression in brain.The 43-amino acid QRFP peptide, a longer form of the P518 peptide is necessary to exhibit full agonistic activity with GPR103. Intravenous administration QRFP caused release of aldosterone, suggesting that QRFP and GPR103 regulate adrenal function.The deduced 126-amino acid protein contains a putative N-terminal 22-amino acid signal peptide and no transmembrane domain, suggesting that the protein or cleavage products can be secreted. The 26-amino acid P518 peptide sequence is located at the C terminus of the precursor protein. In peripheral tissues, P518 precursor mRNA was detected in prostate, testis, colon, thyroid, parathyroid, coronary artery, and bladder.
