Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PXK in samples. An antibody specific for PXK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The deduced 578-amino acid human PXK and 581-amino acid mouse Pxk share over 90% amino acid identity. PXK contains an N-terminal PX-like domain predicted to bind phosphoinositides, a putative protein kinase domain in the central region, which has greatest similarity to Drosophila slob, and a number of polyproline motifs. The authors identified a truncated PXK isoform in both human and mouse. RT-PCR analysis detected expression of the full-length Pxk in all mouse tissues examined and expression of the Pxk short isoform in all mouse tissues except skeletal muscle. Immunohistochemical studies detected Pxk expression throughout the nervous system in both mouse neurons and glia, including oligodendrocytes, astrocytes, microglia, and Schwann cells.
