Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTTG1 in samples. An antibody specific for PTTG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTTG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTTG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTTG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Securin is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
