Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTRH2 in samples. An antibody specific for PTRH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTRH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTRH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTRH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Loss of cell attachment to the extracellular matrix causes apoptosis, a process known as anoikis. BCL2 is an antiapoptotic protein that can protect cells against anoikis. BIT1 is evolutionarily conserved from bacteria to human. It contains an N-terminal mitochondria localization sequence and a C-terminal UPF0099 domain. Northern blot analysis detected a 1-kb BIT1 transcript expressed prominently in testis, prostate, skeletal muscle, and liver, with lower expression in heart, spleen, placenta, and colon. No significant expression was detected in thymus or peripheral leukocytes. Human embryonic kidney cells showed only trace expression of BIT1. Immunostaining localized endogenous BIT1 to the mitochondria of HeLa cells, and immunoblot analysis revealed a 20-kD doublet in HeLa cell mitochondrial extracts.
