Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTPRT in samples. An antibody specific for PTPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:T is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP (MAM) domain, Ig-like and fibronectin type III-like repeats. The protein domain structure and the expression pattern of the mouse counterpart of this PTP suggest its roles in both signal transduction and cellular adhesion in the central nervous system. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Organism species: Homo sapiens (Human)
