Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTPN14 in samples. An antibody specific for PTPN14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPN14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPN14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPN14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Tyrosine-protein phosphatase non-receptor type 14 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation.
This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. The specific function of this PTP has not yet been determined.Expressed in a variety of human tissues including kidney, skeletal muscle, lung and placenta.
