Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTK2B in samples. An antibody specific for PTK2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Protein tyrosine kinase 2 beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2.
