Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTGIR in samples. An antibody specific for PTGIR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGIR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGIR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGIR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Prostacyclin receptor is a member of the G-protein coupled receptor family. Prostacyclin, the major product of cyclooxygenase in macrovascular endothelium, elicits a potent vasodilation and inhibition of platelet aggregation through binding to this receptor.
When binding a prostacyclin-molecule, the receptor changes conformation and activates Gs, with its activation of cAMP and increase in protein kinase A (PKA) activity.In vasodilation, the PKA activity causes phosphorylation of MLCK, decreasing its activity, resulting in dephosphorylation of MLC of myosin. The smooth muscle relaxation leads to vasodilation.
