Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTAFR in samples. An antibody specific for PTAFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTAFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTAFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTAFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The platelet-activating factor receptor is a G-protein coupled receptor which binds platelet-activating factor.The PAF receptor shows structural characteristics of the rhodopsin gene family and binds platelet-activating factor (PAF). PAF is a phospholipid (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) that has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes.
PTAFR has 2 N-glycosylation sites, but unlike other rhodopsin family members, there is no N-glycosylation site in the N-terminal segment. Transfection of PTAFR into COS-7 cells resulted in expression of the receptor with an extracellular N terminus.
