Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PSG9 in samples. An antibody specific for PSG9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSG9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSG9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSG9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy. PSGs comprise a subgroup of the carcinoembryonic antigen (CEA) family, which belongs to the immunoglobulin superfamily. The PSG genes are tandemly oriented in a 5-prime to 3-prime direction from telomere to centromere. The CEA subgroup gene CGM11 is located at the telomeric end of the PSG gene cluster, and 6 genes belonging to a third CEA family subgroup, namely CGM13 through CGM18, are interspersed among the PSG genes.Based on this nomenclature, the CEA family is composed of the PSG subfamily, the CEACAM subfamily, and the CEACAM pseudogene (CEACAMP) subfamily. PSG11, PSG12, and PSG13 were renamed PSG9, PSG10, and PSG11, respectively.
