Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PSG6 in samples. An antibody specific for PSG6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSG6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSG6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSG6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy. PSGs comprise a subgroup of the carcinoembryonic antigen (CEA) family, which belongs to the immunoglobulin superfamily.
PSG10, which had been thought to be a novel gene, is in fact an allelic variant of PSG6 that differs by 10 bp in the coding region.The PSG genes are tandemly oriented in a 5-prime to 3-prime direction from telomere to centromere. The CEA subgroup gene CGM11 is located at the telomeric end of the PSG gene cluster, and 6 genes belonging to a third CEA family subgroup, namely CGM13 through CGM18 (later found to be pseudogenes), are interspersed among the PSG genes.
