Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PSG2 in samples. An antibody specific for PSG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The human pregnancy-specific glycoproteins (PSGs) are a family of proteins that are synthesized in large amounts by placental trophoblasts and released into the maternal circulation during pregnancy. Molecular cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily of genes.
Members of the CEA family consist of a single N domain, with structural similarity to the immunoglobulin variable domains, followed by a variable number of immunoglobulin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif, which has been shown to function as an adhesion recognition signal for several integrins, in the N-terminal domain .
