Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PSAT1 in samples. An antibody specific for PSAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PSAT catalyzes the second step in the pathway, conversion of 3-phosphohydroxypyruvate into 3-phosphoserine. The full-length PSAT-beta transcript encodes a deduced 370-amino acid protein with a calculated molecular mass of 40 kD. PSAT-alpha lacks exon 8 and encodes a deduced 324-amino acid protein with a calculated molecular mass of 35.2 kD. Compared with PSAT-beta, PSAT-alpha lacks 46 amino acids. Both proteins contain a conserved binding domain for the cofactor pyridoxal 5-prime-phosphate (vitamin B6). PSAT-beta shares 92.4% amino acid similarity with its mouse homolog. PSAT-beta orthologs were present in all species examined, including plants, insects, and bacteria.
