Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PRKCZ in samples. An antibody specific for PRKCZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Protein kinase C, zeta is an enzyme which in humans is encoded by the PRKCZ gene. The PRKCZ gene encodes at least two alternative transcripts, the full-length PKC zeta and an N-terminal truncated form PKM zeta. The truncated PKMζ is thought to be responsible for maintaining long-term memories in the brain.PKC-zeta has an N-terminal regulatory domain, followed by a hinge region and a C-terminal catalytic domain. Second messengers stimulate PKCs by binding to the regulatory domain, translocating the enzyme from cytosol to membrane, and producing a conformational change that removes autoinhibition of the PKC catalytic activity. PKM-zeta, a brain-specific isoform of PKC-zeta generated from an alternative transcript, lacks the regulatory region of full-length PKC-zeta and is therefore constitutively active.
