Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PPP1CB in samples. An antibody specific for PPP1CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. PP1 has 3 catalytic subunits, designated alpha, beta, and gamma. PP1-beta is also referred to as PP1-delta. PPP1Cb is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory.
