Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PON1 in samples. An antibody specific for PON1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPON1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PON1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PON1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The paraoxonase (PON) gene family includes 3 genes, PON1, PON2, and PON3, aligned next to each other on chromosome 7. PON1 (EC 3.1.1.2) hydrolyzes the toxic oxon metabolites of several organophosphorous insecticides, including parathion, diazinon, and chlorpyrifos, as well as nerve agents, such as sarin and soman. PON1 also hydrolyzes aromatic esters, preferably those of acetic acid. In addition, PON1 hydrolyzes a variety of aromatic and aliphatic lactones, and it also catalyzes the reverse reaction, lactonization, of gamma- and delta-hydroxycarboxylic acids. Human PON1 is synthesized in liver and secreted into blood, where it is associated exclusively with high density lipoproteins (HDLs) and may protect against development of atherosclerosis (Draganov et al., 2005).
