Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate POLD1 in samples. An antibody specific for POLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
The enzyme is 94% identical to bovine DNA polymerase delta and contains the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contains 2 putative zinc finger domains in the C-terminal region of the molecule as well as a putative nuclear localization signal at the N-terminal end.WRN functionally interacts with DNA polymerase delta, which is required for DNA replication and DNA repair.
