Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PNLIPRP1 in samples. An antibody specific for PNLIPRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNLIPRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNLIPRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNLIPRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PNLIPRP1 has 68% amino acid sequence identity with PNLIP, 63% identity with PNLIPRP2, and 18 to 34% identity with gastric lipase), lipoprotein lipase, and hepatic lipase . Western blot analysis of recombinant PNLIPRP1 expressed in mammalian cells detected a secreted 47-kD protein. Recombinant PNLIPRP1 did not have lipolytic activity in a standard pancreatic lipase assay, suggesting that PNLIPRP1 may have different substrate and/or cofactor requirements than those present in the assay. Northern blot analysis of pancreas RNA detected a major 1.8-kb PNLIPRP1 transcript.
The expression level of PNLIPRP1 in pancreas was approximately 4-fold lower and 6-fold higher than the expression levels of PNLIP and PNLIPRP2, respectively.
