Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PMVK in samples. An antibody specific for PMVK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMVK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMVK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMVK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PMVK (EC 2.7.4.2) is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate as the fifth reaction of the cholesterol biosynthetic pathway. The deduced 192-amino acid PMVK protein has a calculated molecular mass of about 22 kD. It contains a C-terminal peroxisomal targeting sequence, and a single methionine is removed from the N terminus upon maturation of the protein. Expression was highest in heart and skeletal muscle, with slightly lower levels in liver, kidney, and pancreas, and low but detectable levels in brain, lung, and placenta.Analysis of transcripts from human lymphoblasts subcultured in lipid-depleted sera (LDS) and LDS supplement with lovastatin indicated that PMVK expression is subject to regulation by sterol at the level of transcription.
