Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PMCH in samples. An antibody specific for PMCH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMCH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMCH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMCH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).
