Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLS3 in samples. An antibody specific for PLS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus.Alternate splicing results in multiple transcript variants.
