Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLS1 in samples. An antibody specific for PLS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11. The predicted 629-amino acid protein is 86%, 75%, and 73% identical to chicken fimbrin, human T-plastin (PLS3), and L-plastin (LCP1), respectively. I-plastin migrated as a 68-kD protein on Western blots of human small intestine and colon extracts.
