Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLD4 in samples. An antibody specific for PLD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Phospholipase D (PLD) catalyzes conversion of phosphatidylcholine into choline and phosphatidic acid, leading to a variety of intracellular signal transduction events.
PLD4 officially belongs to the PLD family, because it possesses two HKD motifs. However, it lacks PX and PH domains and has a putative transmembrane domain instead. Nevertheless, little is known regarding expression, structure, and function of PLD4.PLD4 is a non-PLD, HKD motif-carrying, transmembrane glycoprotein localized in the endoplasmic reticulum and Golgi apparatus. The spatiotemporally restricted expression patterns suggested that PLD4 might play a role in common function(s) among microglia during early postnatal brain development and splenic marginal zone cells.
