Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLD2 in samples. An antibody specific for PLD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control. Northern blot analysis demonstrated expression of an approximately 3.5-kb PLD2 transcript in various tissues including heart, placenta, pancreas, spleen, thymus, prostate, uterus, brain, lung, kidney, small intestine, and colon. Expression was relatively low in liver and skeletal muscle. An additional 3.8-kb transcript was expressed in peripheral blood leukocytes.
