Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLAC9 in samples. An antibody specific for PLAC9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAC9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAC9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAC9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By microarray assay to search for mouse genes enriched in placenta, Galaviz-Hernandez et al. (2003) identified Plac9 as a placenta-enriched gene expressed in mouse 16-cell morula cDNA. By database analysis, they identified human PLAC9, which encodes a deduced 97-amino acid protein. The deduced 108-amino acid mouse protein has a predicted signal peptide, a coiled-coil domain, and shares 73% identity with human PLAC9. Northern blot analysis of mouse placenta and embryo RNA at 12.5 and 18.5 days postcoitum (dpc) detected predominant expression in placenta.Galaviz-Hernandez et al. (2003) determined that the mouse Plac9 gene contains 4 exons, with exons 4a and 4b defining different Plac9 isoforms. Galaviz-Hernandez et al. (2003) mapped the PLAC9 gene to chromosome 10q22.3 and the mouse Plac9 gene to chromosome 15.
