Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLA2G6 in samples. An antibody specific for PLA2G6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:85 kDa calcium-independent phospholipase A2 is is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date.Tang et al. (1997) purified an 85-kD cytosolic calcium-independent PLA2, termed iPLA2, from Chinese hamster ovary (CHO) cells. They isolated a partial human cDNA from a Burkitt lymphoma cDNA library by screening with a CHO cDNA. Northern blot analysis revealed that iPLA2 is expressed in a variety of rodent tissues.
