Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLA2G4D in samples. An antibody specific for PLA2G4D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G4D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G4D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G4D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PLA2G4D acts as a calcium-dependent phospholipase A2 enzyme with a lineolic acid-specific activity. Using in situ hybridization and immunohistochemistry of normal and psoriatic skin, as well as skin from patients with inflammatory skin conditions such as atopic dermatitis and mycosis fungoides, the authors determined that PLA2G4D mRNA and protein display a strong specific expression in the upper epidermis in psoriatic skin, and not in lower epidermis and dermis.
A similar distribution of PLA2G4D expression, at decreased levels, was detected in the upper epidermis of patients with other inflammatory skin conditions, while PLA2G4D mRNA and protein were not detected in normal adult skin.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases.
