Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLA2G4A in samples. An antibody specific for PLA2G4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Cytosolic phospholipases A2 (cPLA2): The intracellular PLA2 are also Ca-dependent, but they have completely different 3D structure and significantly larger than secreted PLA2 (more than 700 residues). They include C2 domain and large catalytic domain. These phospholipases are involved in cell signaling processes, such as inflammatory response. The produced Arachidonic acid is both a signaling molecule and the precursor for other signalling molecules termed eicosanoids. These include leukotrienes and prostaglandins. Some eicosanoids are synthesized from diacylglycerol, released from the lipid bilayer by phospholipase C (see below). Phospholipases A2 can be classified based on sequence homology.PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands
