Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLA2G2D in samples. An antibody specific for PLA2G2D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G2D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G2D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G2D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PLA2G2D, The extracellular forms of phospholipases A2 have been isolated from different venoms (snake, bee, and wasp), from virtually every studied mammalian tissue (including pancreas and kidney) as well as from bacteria. They require Ca2+ for activity.Pancreatic PLA2 serve for the initial digestion of phospholipid compounds in dietary fat. Venom phospholipases help to immobilize prey by promoting cell lysis.Analysis of enzymatic activity detected most activity in culture supernatant and determined that PLA2G2D preferentially hydrolyzes phosphatidylglycerol and phosphatidylethanolamine, followed by phosphatidylcholine, but does not hydrolyze phosphatidylserine or phosphatidic acid. Northern blot analysis revealed variable expression of 2.0- and 1.0-kb transcripts, with highest expression in pancreas and spleen.
