Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLA2G1B in samples. An antibody specific for PLA2G1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Phospholipase A2 catalyzes the release of fatty acids from glycero-3-phosphocholines. The Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets.In analogous human cDNA obtained from human lung, they also obtained and analyzed the apparently unique human gene encoding pancreatic PLA2. Hybridization probes made from human 'pancreatic' cDNA hybridized to two distinct genomic fragments of cobra DNA, indicating again a striking homology to one or more counterpart in one variety of snake.
