Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PIP5K1C in samples. An antibody specific for PIP5K1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIP5K1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIP5K1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIP5K1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PIP5K1g encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.Pip5k1c has 2 alternative splice forms, consisting of 635 and 661 amino acids, that migrated at 87 and 90 kD by SDS-PAGE, respectively. The isoforms differ by the inclusion or exclusion of a 26-amino acid C-terminal tail. Northern blot analysis detected high levels of Pip5k1c expression in mouse brain, lung, and kidney.
AP2 via its mu-2 subunit (AP2M1) directly interacted with the kinase core domain of the PIPK gamma-subunit (PIP5K1C) in vitro and in native protein extracts. Endocytic cargo protein binding to mu-2 stimulated PIPK activity.
