Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PHF7 in samples. An antibody specific for PHF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHF7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHF7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, PHF7 is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis.
