Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PGM3 in samples. An antibody specific for PGM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The interaction of erythropoietin (EPO) with the EPO receptor (EPOR) activates multiple signaling cascades. The deduced 542-amino acid AGM1 protein is identical to human N-acetylglucosamine-phosphate mutase . It contains a 10-amino acid segment showing high similarity with the putative active site motif of several hexose phosphate mutases.
Northern blot analysis detected a major, approximately 2.4-kb AGM1 transcript in all human tissues tested except lung, with relatively high expression in pancreas, heart, liver, and placenta, and relatively low expression in brain, skeletal muscle, and kidney. Two minor transcripts of approximately 5 and 8 kb were also found in all tissues except lung.
