Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PGLYRP2 in samples. An antibody specific for PGLYRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGLYRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGLYRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGLYRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The deduced 576-amino acid precursor protein contains an N-terminal signal peptide, followed by 2 transmembrane segments and an extracellular C terminus containing PGRP domains III, II, and I. PGRPL shares 40%, 33%, and 32% identity with PGRPS, PGRPI-alpha, and PGRPI-beta, respectively. RNA dot blot analysis detected strong PGRPL expression in adult liver and weak expression in fetal liver. Northern blot analysis detected 2.1- and 0.8-kb transcripts expressed in adult and fetal liver. PCR also detected low expression in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach, and testis. Transiently transfected COS-7 and human embryonic kidney cells expressed PGRPL as a Triton X-100-soluble membrane protein with an apparent molecular mass of 65 kD.
