Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PFDN5 in samples. An antibody specific for PFDN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFDN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFDN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFDN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Prefoldin subunit 5 is a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described.Fluorescent microscopy showed MM1 expression primarily in the nucleus, with lower intensity in nucleoli and cytoplasm. Binding analyses indicated that MM1 and c-myc bind directly and that all but the N-terminal 13 amino acids of MM1 are required for binding.
